We detail the effect on the performance and costs of each parameter, design, and hardware choice, guiding readers toward the realization of optimized devices that can be employed out of the lab by non-expert users for point of care testing.This paper addresses retinal vessel segmentation on optical coherence tomography angiography (OCT-A) images of the human retina. https://www.selleckchem.com/products/doxycycline.html Our approach is motivated by the need for high precision image-guided delivery of regenerative therapies in vitreo-retinal surgery. OCT-A visualizes macular vasculature, the main landmark of the surgically targeted area, at a level of detail and spatial extent unattainable by other imaging modalities. Thus, automatic extraction of detailed vessel maps can ultimately inform surgical planning. We address the task of delineation of the Superficial Vascular Plexus in 2D Maximum Intensity Projections (MIP) of OCT-A using convolutional neural networks that iteratively refine the quality of the produced vessel segmentations. We demonstrate that the proposed approach compares favourably to alternative network baselines and graph-based methodologies through extensive experimental analysis, using data collected from 50 subjects, including both individuals that underwent surgery for structural macular abnormalities and healthy subjects. Additionally, we demonstrate generalization to 3D segmentation and narrower field-of-view OCT-A. In the future, the extracted vessel maps will be leveraged for surgical planning and semi-automated intraoperative navigation in vitreo-retinal surgery.In this paper, we study a photonic Bragg waveguide sensor for resonant sensing applications in the THz range. In order to enhance the resolution and detectivity of the sensor, we modify the relatively broad transmission spectrum of the Bragg waveguide with spectrally narrow transmission dips by creating a geometrical defect in Bragg reflector and causing anti-crossing phenomenon between the core-guided mode and defect mode. The spectral position of the resonant dip is highly sensitive to the thickness variation in the vicinity of the waveguide core. By designing and manufacturing a Bragg waveguide which includes several sections with different defect layer thicknesses, we can interrogate more than one sample simultaneously and thereby realize multichannel resonant sensing by directly tracking the independent resonant dips. Furthermore, we demonstrate the waveguide platform for online monitoring of the thickness variation of lactose powders, which is captured on the waveguide core via a centrifugal force using a home-built rotating setup. Additionally, we also demonstrate the waveguide for fingerprint detection of powder analytes, which further enriches the sensing scenario of the sensing platform. Finally, we discuss the advantages and the spectral tailoring flexibility of the THz Bragg waveguides sensors for future implementations.Photoacoustic microscopy and macroscopy (PAM) using focused detector scanning are emerging imaging methods for biological tissue, providing high resolution and high sensitivity for structures with optical absorption contrast. However, achieving a constant lateral resolution over a large depth of field for deeply penetrating photoacoustic macroscopy is still a challenge. In this work, a detector design for scanning photoacoustic macroscopy is presented. Based on simulation results, a sensor array geometry is developed and fabricated that consists of concentric ring elements made of polyvinylidene fluoride (PVDF) film in a geometry that combines a centered planar ring with several inclined outer ring elements. The reconstruction algorithm, which uses dynamic focusing and coherence weighting, is explained and its capability to reduce artefacts occurring for single element conical sensors is demonstrated. Several phantoms are manufactured to evaluate the performance of the array in experimental measurements. The sensor array provides a constant axial and lateral resolution of 95 ?m and 285 ?m, respectively, over a depth of field of 20 mm. The depth of field corresponds approximately to the maximum imaging depth in biological tissue, estimated from the sensitivity of the array. With its ability to achieve the maximum resolution even with a very small scanning range, the array is believed to have applications in the imaging of limited regions of interest buried in biological tissue.Optical microscopes are an essential tool for both the detection of disease in clinics, and for scientific analysis. However, in much of the world access to high-performance microscopy is limited by both the upfront cost and maintenance cost of the equipment. Here we present an open-source, 3D-printed, and fully-automated laboratory microscope, with motorised sample positioning and focus control. The microscope is highly customisable, with a number of options readily available including trans- and epi- illumination, polarisation contrast imaging, and epi-florescence imaging. The OpenFlexure microscope has been designed to enable low-volume manufacturing and maintenance by local personnel, vastly increasing accessibility. We have produced over 100 microscopes in Tanzania and Kenya for educational, scientific, and clinical applications, demonstrating that local manufacturing can be a viable alternative to international supply chains that can often be costly, slow, and unreliable.This study aimed to evaluate the variations of infrared thermography according to rapid hemodynamic changes, by measuring the peripheral skin temperature in a porcine model. Eight healthy piglets were anesthetized and exposed to different levels of arterial pressure. Thermography was performed on the left forelimb to measure carpus and elbow skin temperature and their associated gradient with the core temperature. Changes in skin temperature in response to variations of blood pressure were observed. A negative correlation between arterial pressure and temperature gradients between peripheral and core temperature and a negative correlation between cardiac index and these temperature gradients were observed. Thermography may serve as a tool to detect early changes in peripheral perfusion.